In a series of tests were conducted 30 mins before and 30 mins after Agnihotra, at various location in India. It was noted Agnihotra performance had a marked influence on the atmospheric values. The PM10 and PM 2.5 values decreased, levels of Carbon Monoxide and Carbon Di Oxide were reduced, Oxygen levels stabilized and the levels of other pollutants like Oxides of Nitrogen and Ammonia were also reduced.